Fish Cloning And Catfish Hatching
Introduction To Fish Cloning And Catfish Hatching
Similarly, Cloning in Biotechnology refers to processes used to create copies of DNA fragments (molecular cloning), cells (cell cloning), or organisms. Molecular cloning refers to the process of making multiple molecules, cloning is commonly used to amplify DNA fragments containing whole genes, but it can also be used to amplify any DNA sequence such as promoters, non-coding sequence and randomly fragmented DNA. It is used in a wide array of biological experiments and practical applications ranging from genetic fingerprint to large scale protein production.
More on Fish Cloning And Catfish Hatching
Using Zebra fish as example; Zebra fish served as an excellent model for understanding normal development and birth defects. Likewise, research with Zebra fish has extended to model human diseases and to analyze the formation and functions of cell populations with organs. The normal cloning
technique uses an unfertilized egg and a donor cell. The DNA is removed from
the egg and replaced from the donor. The egg is then coaxed into dividing the resulting fish is an exact genetic copy of the donor.
Furthermore, using Gibelli and colleges technique, the egg is gently removed from the female Zebra fish and placed in a solution of ovarian fluyid from a chinnok salmon. This worked well, because it kept the egg inactive for sometime in about two or three hours. Zebra fish was useful in the exercise because their eggs are transparent and the fish development is easy to follow.
Though reproductive cloning generally uses somatic cell nuclear transfer (SCNT) to create organisms that are generally identical. This process entails the transfer of a nucleus from a donor adult cell (somatic cell) to an egg from which the nucleus has been removed, or to a cell from a blast cyst. If the egg begins to divide normally, it is transferred into the uterus of the surrogate mother. Additionally the mitochondria in the cytoplasm also contains DNA and during SCNT, this mitochondrial DNA is wholly from the cytoplasm donor’s egg, thus the mitochondrial genome is not the same as that of the nucleus donor cell from which it was produced. This may have important implications split for cross species nuclear transfer in which nuclear mitochondrial incompatibilities may lead to death.Furthermore, artificial embryo splitting or embryo twinning, a technique that creates monozygotic twins from a single embryo is not considered in the same fashion as other methods of cloning. During the procedure, a donor embryo is split in two distinct embryos that can then be transferred via embryo transfer. It is optimally performed at the 6 to 8 cell stage, where it can be used as an expansion of IVF to increase the number of available embryos. Likewise, if both embryos are successful, it gives rise to monozygotic (identical) twins.
- The exercise produces new offspring that are genetically identical, they will all have the desired characteristics.
- Similarly, Organisms that are difficult or slow to breed normally reproduced quickly.
- Firstly, if a clone is susceptible to disease or changes in the environment, then all the clones will be susceptible.
- Secondly, it will lead to less variation, and less opportunity to create new varieties in future.
SIGNIFICANCE OF FISH CLONING IN BIOLOGY AND BIOTECHNOLOGY
Firstly, molecular cloning allows scientists to not only discover what proteins are present and their function, but also explore what happens in a cell wall when these proteins are changed. When studying cell divisions, specifically, scientists look for proteins that control the beginning and end of division, even the replication within the cell, example; Histones.
By manipulating cells with cloning and learning more about specific proteins, scientists can take their research and apply it to large- sale research endeavors like diseases and pathogens.
Also, it is vital to note, however, the cloning is not used to study just proteins involved in the cell cycle. Molecular cloning has lead scientists to discover the entire genetic sequences of many different species, inactivate genes in both human and other organisms and create transgenic like herbicide resistant plants and glow- in- the dark fish. Intuitively, when tropical fish give off a slight glow because they have been marked with the same fluorescence that is native to some species of jellyfish.
- Textbook of somatic cell nuclear transfer- Embryo twinning. By; Hans Diriesh (1885), Article on cloning- Star Garden (April 2014).
- Article on MSU Researchers- Improve cloning method for zebra fish. By; Jose Cibelli and Tom Oswald (August 2009).
- Article on Nature methods. By; G L. Kohuth (Michigan state university- September 2009).
- Textbook on Heat shock genes and their functional significance in fish. By; A E. Todgham, P A. Ackerman, George K. Iwama (2002).
Contact Us Today, Let’s Help You Prepare An Irresistible Business Plan And A Feasibility Study Report
Do you need a business plan to raise fund for your business?
We can carry out research and develop MBA grade business plans that you can present to a bank or investor. Intuitively, Chris Farm Nigeria develops well self-explanatory, irresistible feasibility studies or business plan for your Business start ups, Business Growth or Expansions through either personal funds, Grants, or loans, which could be new or existing ones. We delight in writing for people under Academic sectors, production sectors, manufacturing sectors, processing or packing sectors, advertising sectors, marketing sectors and other related sectors on mini, middle and large scale businesses.
For your Agribusiness, either production, processing, marketing of any Agro-industry, our feasibility studies or business plan are explicit.
General Info On Feasibility Study And Business Plans
Intuitively, our feasibility study and business plans are explicit. Likewise, becomes irresistible when you show them to your investors or sponsors. It gives you a clear picture of what you are to see. Especially, when you put the feasibility study or business plans into use.
More Info On Feasibility Study And Business Plans
Similarly, it shows how much it will cost you to own your desired business, what your money can afford. The kinds of product you will need to minimize input in other to maximize output. Likewise, how much returns you will get at the end of each accounting year until your business break-even on its initial investment capital. Lastly, this will give the intending business person or investor or farmer, a vivid idea on the possible benefit he or she stands to gain, when he or she starts doing the business. However, the intending business person or investor or farmer will not need a soothsayer to make decision for him or her.
CLICK HERE to see what our Business Plans and Feasibility Study Reports has for you.
Intuitively, Our Business Plans and Feasibility Study Report has a unique content. Take a look at our it. Finally, be rest assured that we give you what you want if you place and order. For easy understand of your idea, Call us or send us an email for your request. And you will be Glad you did.
Pay For Any Service Via Clicking PAYMENT On the Menu
Finally, Know more about Catfish Farming Business by ordering for this book for just Ten thousand Naira (N10,000.00)
PRICE TAG: N10, 000.00
Mobile Fish Ponds For Sale All Over Nigeria
Furthermore, Fish Collapsible Tarpaulin Ponds Tips is a Simple guide For All Fish Farmers. As a matter of fact, Chris farm Nigeria welcomes you on Fish Ponds or Collapsible Tarpaulin Ponds Section. This is where we talk about Modern Fish Farming. Join our Facebook Niche Here